antibody rabbit polyclonal anti-vglut1  (Synaptic Systems)

Journal: eLife

Article Title: Translatome analysis reveals cellular network in DLK-dependent hippocampal glutamatergic neuron degeneration

doi: 10.7554/eLife.101173

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Article Snippet: Antibody , Rabbit polyclonal anti-Vglut1 , Synaptic Systems , 135 302; RRID: AB_887877 , IF (1:1000) tissue; Lot #1–53.

Techniques: Knock-Out, Over Expression, Sequencing, RNAscope, TUNEL Assay, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Multiplex Assay, Software

Journal: iScience

Article Title: Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons

doi: 10.1016/j.isci.2023.106285

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Article Snippet: Rabbit polyclonal anti-VGLUT1 , Proteintech , Cat# 55491-1-AP; RRID: AB_2881348.

Techniques: Control, Recombinant, Membrane, Clone Assay, Reverse Transcription, RNA HS Assay, DNA Library Preparation, Expressing, Derivative Assay, Software, Microscopy, Cell Culture

Journal: iScience

Article Title: Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes

doi: 10.1016/j.isci.2022.104762

Figure Lengend Snippet: Establishment of an autaptic culture with human iPSC-derived astrocytes (A) The protocol for the generation of human iPSC-derived astrocytes (HiAs). (B) mRNA levels of glial fibrillary acidic protein ( Gfap ), CD44 , and SOX1 in Lt-NES cells or HiAs (n = number of cultures: Lt-NES cells, n = 8; HiAs, n = 8; two experiments). Data are represented as mean ± SEM (Student’s t test, ∗p < 0.05, ∗∗∗p < 0.001). (C) Representative images of HiAs immunostained for GFAP (red) and S100β (green). Scale bar 50 μm. (D) Percentage of GFAP-positive cells in HiAs (n = number of cultures: n = 7; two experiments). Data are represented as mean ± SEM. (E) The protocol of the autaptic culture with HiAs (HiAACs). (F) Representative images of HiAACs immunostained for GFAP (red) and microtubule-associated protein 2 (MAP2; green). Scale bar 50 μm.

Article Snippet: Rabbit anti-GFAP polyclonal antibody , Synaptic Systems , Cat# 173 002 RRID: AB_2864794.

Techniques: Derivative Assay

Journal: iScience

Article Title: Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes

doi: 10.1016/j.isci.2022.104762

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Article Snippet: Rabbit anti-GFAP polyclonal antibody , Synaptic Systems , Cat# 173 002 RRID: AB_2864794.

Techniques: Recombinant

Journal: iScience

Article Title: Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes

doi: 10.1016/j.isci.2022.104762

Figure Lengend Snippet: Neurons in HiAACs develop morphologically and form excitatory synapses (A) Representative images of single neurons in NMC or HiAAC immunostained for vesicular glutamate transporter 1 (VGlut1; red) and MAP2 (green). Parts of the top row (scale bar 50 μm) are enlarged in the bottom row (scale bar 10 μm). (B) Mean number of VGlut1–positive synaptic puncta in single neurons in NMC or HiAAC. (C) Mean total dendritic length in single neurons in NMC or HiAAC. (D) Mean number of dendritic branches in single neurons in NMC or HiAAC. (E) Correlation between dendritic length and number of synapses in HiAACs (left) and NMCs (right). The x axis is the total dendritic length and the y axis is the number of VGlut1–positive synaptic puncta. n = number of neurons: NMCs, n = 37; HiAACs, n = 34; two cultures. All data are represented as mean ± SEM (Student’s t test, ∗p < 0.05, ∗∗∗p < 0.001).

Article Snippet: Rabbit anti-VGLUT1 polyclonal antibody , Synaptic Systems , Cat# 135 303 RRID: AB_2744597.

Techniques:

Journal: iScience

Article Title: Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes

doi: 10.1016/j.isci.2022.104762

Figure Lengend Snippet: HiAACs mature from 2 to 4 weeks in culture and can be used for experiments for at least 6 weeks in vitro (A) Representative traces of evoked EPSCs recorded from HiAACs neurons cultured for 2, 4, or 6 weeks (WIV2, WIV4, and WIV6) by the time of electrophysiological recording. (B) Mean amplitudes of the evoked EPSCs in HiAAC neurons of WIV2, WIV4, and WIV6 (n = number of neurons: WIV2, n = 35; WIV4, n = 33; WIV6, n = 29; four cultures). (C) Representative mEPSC traces in HiAAC neurons of WIV2, WIV4, and WIV6. (D and E) Mean mEPSC frequencies and amplitudes in HiAAC neurons of WIV2, WIV4, and WIV6 (n = number of neurons: WIV2, n = 32; WIV4, n = 33; WIV6, n = 28; four cultures). (F) Representative images of HiAAC neurons of WIV2, WIV4, or WIV6 immunostained for the dendritic marker MAP2 (green) and the excitatory nerve terminal marker VGlut1 (red). Parts of the top row (scale bar 50 μm) are enlarged in the bottom row (scale bar 10 μm). (G) Mean number of VGlut1–positive synaptic puncta in HiAAC neurons of WIV2, WIV4, and WIV6 (n = number of neurons: WIV2, n = 34; WIV4, n = 28; WIV6, n = 24; two cultures). All data are represented as mean ± SEM (Tukey’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

Article Snippet: Rabbit anti-VGLUT1 polyclonal antibody , Synaptic Systems , Cat# 135 303 RRID: AB_2744597.

Techniques: In Vitro, Cell Culture, Marker

Journal: iScience

Article Title: Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes

doi: 10.1016/j.isci.2022.104762

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Article Snippet: Rabbit anti-VGLUT1 polyclonal antibody , Synaptic Systems , Cat# 135 303 RRID: AB_2744597.

Techniques: Recombinant

Journal: iScience

Article Title: Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKΙΙα-Cre mouse line

doi: 10.1016/j.isci.2022.104692

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Article Snippet: Rabbit polyclonal antibody anti-VGluT1 , Millipore , Cat#ABN1647; RRID: AB_2814811.

Techniques: Virus, Recombinant, cDNA Synthesis, Western Blot, Stripping, Immunodetection, Plasmid Preparation, Isolation, Sequencing, Software